The S28H mutation on mNeptune generates a brighter near-infrared monomeric fluorescent protein with improved quantum yield and pH-stability.
Acta Biochim Biophys Sin (Shanghai)
; 46(9): 802-9, 2014 Sep.
Article
em En
| MEDLINE
| ID: mdl-25062707
ABSTRACT
For living deep-tissue imaging, the optical window favorable for light penetration is in near-infrared wavelengths, which requires fluorescent proteins with emission spectra in the near-infrared region. Here, we report that a single mutant Ser28His of mNeptune with a near-infrared (≥650 nm) emission maxima of 652 nm is found to improve the brightness, photostability, and pH stability when compared with its parental protein mNeptune, while it remains as a monomer, demonstrating that there is still plenty of room to improve the performance of the existing near infrared fluorescence proteins by directed evolution.
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Coleções:
01-internacional
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MEDLINE
Assunto principal:
Teoria Quântica
/
Espectroscopia de Luz Próxima ao Infravermelho
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Concentração de Íons de Hidrogênio
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Proteínas Luminescentes
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Mutação
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article