Increased expression of SAMHD1 in a subset of HIV-1 elite controllers.
J Antimicrob Chemother
; 69(11): 3057-60, 2014 Nov.
Article
em En
| MEDLINE
| ID: mdl-25063780
ABSTRACT
OBJECTIVES:
SAMHD1 and the CDKN1A (p21) cyclin-dependent kinase inhibitor have been postulated to mediate HIV-1 restriction in CD4+ cells. We have shown that p21 affects HIV replication through its effect on SAMHD1. Thus, we aimed at evaluating the expression of SAMHD1 and p21 in different HIV+ phenotypic groups. PATIENTS ANDMETHODS:
We evaluated SAMHD1 and CDKN1A mRNA expression in CD4+ T cells from HIV+ individuals including elite controllers (nâ=â12), individuals who control HIV without the need for antiretroviral treatment, viraemic progressors (nâ=â10) and HIV-1 seronegative healthy donors (nâ=â14). Immunological variables were measured by flow cytometry.RESULTS:
We show that a subset of HIV+ elite controllers with lower T cell proliferation levels (Ki67+ cells) expressed higher SAMHD1 compared with healthy donors or viraemic progressors. Conversely, there was no difference in p21 expression before or after T cell activation with a bispecific CD3/CD8 antibody.CONCLUSIONS:
Our results suggest that SAMHD1 may play a role in controlling virus replication in HIV+ individuals and slow the rate of disease progression.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fenótipo
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Replicação Viral
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Regulação Enzimológica da Expressão Gênica
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HIV-1
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Proteínas Monoméricas de Ligação ao GTP
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article