Expression of natural human ß1,4-GalT1 variants and of non-mammalian homologues in plants leads to differences in galactosylation of N-glycans.
Transgenic Res
; 23(5): 717-28, 2014 Oct.
Article
em En
| MEDLINE
| ID: mdl-25082356
ABSTRACT
ß1,4-Galactosylation of plant N-glycans is a prerequisite for commercial production of certain biopharmaceuticals in plants. Two different types of galactosylated N-glycans have initially been reported in plants as the result of expression of human ß1,4-galactosyltransferase 1 (GalT). Here we show that these differences are associated with differences at its N-terminus the natural short variant of human GalT results in hybrid type N-glycans, whereas the long form generates bi-antennary complex type N-glycans. Furthermore, expression of non-mammalian, chicken and zebrafish GalT homologues with N-termini resembling the short human GalT N-terminus also induce hybrid type N-glycans. Providing both non-mammalian GalTs with a 13 amino acid N-terminal extension that distinguishes the two naturally occurring forms of human GalT, acted to increase the levels of bi-antennary galactosylated N-glycans when expressed in tobacco leaves. Replacement of the cytosolic tail and transmembrane domain of chicken and zebrafish GalTs with the corresponding region of rat α2,6-sialyltransferase yielded a gene whose expression enhanced the level of bi-antennary galactosylation even further.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
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Nicotiana
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Biofarmácia
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Engenharia Genética
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Folhas de Planta
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Galactosiltransferases
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article