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Dual function of the cytochrome P450 CYP76 family from Arabidopsis thaliana in the metabolism of monoterpenols and phenylurea herbicides.
Höfer, René; Boachon, Benoît; Renault, Hugues; Gavira, Carole; Miesch, Laurence; Iglesias, Juliana; Ginglinger, Jean-François; Allouche, Lionel; Miesch, Michel; Grec, Sebastien; Larbat, Romain; Werck-Reichhart, Danièle.
Afiliação
  • Höfer R; Institute of Plant Molecular Biology, Centre National de la Recherche Scientifique Unité Propre de Recherche 2357 (R.H., B.B., H.R., C.G., J.I., J.-F.G., D.W.-R.), Institute for Advanced Study (H.R., D.W.-R.), Laboratoire de Chimie Organique Synthétique, Institut de Chimie, Centre National de la Rec
  • Boachon B; Institute of Plant Molecular Biology, Centre National de la Recherche Scientifique Unité Propre de Recherche 2357 (R.H., B.B., H.R., C.G., J.I., J.-F.G., D.W.-R.), Institute for Advanced Study (H.R., D.W.-R.), Laboratoire de Chimie Organique Synthétique, Institut de Chimie, Centre National de la Rec
  • Renault H; Institute of Plant Molecular Biology, Centre National de la Recherche Scientifique Unité Propre de Recherche 2357 (R.H., B.B., H.R., C.G., J.I., J.-F.G., D.W.-R.), Institute for Advanced Study (H.R., D.W.-R.), Laboratoire de Chimie Organique Synthétique, Institut de Chimie, Centre National de la Rec
  • Gavira C; Institute of Plant Molecular Biology, Centre National de la Recherche Scientifique Unité Propre de Recherche 2357 (R.H., B.B., H.R., C.G., J.I., J.-F.G., D.W.-R.), Institute for Advanced Study (H.R., D.W.-R.), Laboratoire de Chimie Organique Synthétique, Institut de Chimie, Centre National de la Rec
  • Miesch L; Institute of Plant Molecular Biology, Centre National de la Recherche Scientifique Unité Propre de Recherche 2357 (R.H., B.B., H.R., C.G., J.I., J.-F.G., D.W.-R.), Institute for Advanced Study (H.R., D.W.-R.), Laboratoire de Chimie Organique Synthétique, Institut de Chimie, Centre National de la Rec
  • Iglesias J; Institute of Plant Molecular Biology, Centre National de la Recherche Scientifique Unité Propre de Recherche 2357 (R.H., B.B., H.R., C.G., J.I., J.-F.G., D.W.-R.), Institute for Advanced Study (H.R., D.W.-R.), Laboratoire de Chimie Organique Synthétique, Institut de Chimie, Centre National de la Rec
  • Ginglinger JF; Institute of Plant Molecular Biology, Centre National de la Recherche Scientifique Unité Propre de Recherche 2357 (R.H., B.B., H.R., C.G., J.I., J.-F.G., D.W.-R.), Institute for Advanced Study (H.R., D.W.-R.), Laboratoire de Chimie Organique Synthétique, Institut de Chimie, Centre National de la Rec
  • Allouche L; Institute of Plant Molecular Biology, Centre National de la Recherche Scientifique Unité Propre de Recherche 2357 (R.H., B.B., H.R., C.G., J.I., J.-F.G., D.W.-R.), Institute for Advanced Study (H.R., D.W.-R.), Laboratoire de Chimie Organique Synthétique, Institut de Chimie, Centre National de la Rec
  • Miesch M; Institute of Plant Molecular Biology, Centre National de la Recherche Scientifique Unité Propre de Recherche 2357 (R.H., B.B., H.R., C.G., J.I., J.-F.G., D.W.-R.), Institute for Advanced Study (H.R., D.W.-R.), Laboratoire de Chimie Organique Synthétique, Institut de Chimie, Centre National de la Rec
  • Grec S; Institute of Plant Molecular Biology, Centre National de la Recherche Scientifique Unité Propre de Recherche 2357 (R.H., B.B., H.R., C.G., J.I., J.-F.G., D.W.-R.), Institute for Advanced Study (H.R., D.W.-R.), Laboratoire de Chimie Organique Synthétique, Institut de Chimie, Centre National de la Rec
  • Larbat R; Institute of Plant Molecular Biology, Centre National de la Recherche Scientifique Unité Propre de Recherche 2357 (R.H., B.B., H.R., C.G., J.I., J.-F.G., D.W.-R.), Institute for Advanced Study (H.R., D.W.-R.), Laboratoire de Chimie Organique Synthétique, Institut de Chimie, Centre National de la Rec
  • Werck-Reichhart D; Institute of Plant Molecular Biology, Centre National de la Recherche Scientifique Unité Propre de Recherche 2357 (R.H., B.B., H.R., C.G., J.I., J.-F.G., D.W.-R.), Institute for Advanced Study (H.R., D.W.-R.), Laboratoire de Chimie Organique Synthétique, Institut de Chimie, Centre National de la Rec
Plant Physiol ; 166(3): 1149-61, 2014 Nov.
Article em En | MEDLINE | ID: mdl-25082892
ABSTRACT
Comparative genomics analysis unravels lineage-specific bursts of gene duplications related to the emergence of specialized pathways. The CYP76C subfamily of cytochrome P450 enzymes is specific to Brassicaceae. Two of its members were recently associated with monoterpenol metabolism. This prompted us to investigate the CYP76C subfamily genetic and functional diversification. Our study revealed high rates of CYP76C gene duplication and loss in Brassicaceae, suggesting the association of the CYP76C subfamily with species-specific adaptive functions. Gene differential expression and enzyme functional specialization in Arabidopsis thaliana, including metabolism of different monoterpenols and formation of different products, support this hypothesis. In addition to linalool metabolism, CYP76C1, CYP76C2, and CYP76C4 metabolized herbicides belonging to the class of phenylurea. Their ectopic expression in the whole plant conferred herbicide tolerance. CYP76Cs from A. thaliana. thus provide a first example of promiscuous cytochrome P450 enzymes endowing effective metabolism of both natural and xenobiotic compounds. Our data also suggest that the CYP76C gene family provides a suitable genetic background for a quick evolution of herbicide resistance.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Família Multigênica / Arabidopsis / Regulação da Expressão Gênica de Plantas / Sistema Enzimático do Citocromo P-450 / Herbicidas Idioma: En Ano de publicação: 2014 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Família Multigênica / Arabidopsis / Regulação da Expressão Gênica de Plantas / Sistema Enzimático do Citocromo P-450 / Herbicidas Idioma: En Ano de publicação: 2014 Tipo de documento: Article