RNA aptamers for targeting mitochondria using a mitochondria-based SELEX method.
Biol Pharm Bull
; 37(8): 1411-5, 2014.
Article
em En
| MEDLINE
| ID: mdl-25087963
ABSTRACT
The use of mitochondria-based systematic evolution of ligands by exponential enrichment (SELEX) was explored. Mitochondria were isolated from rat liver and confirmed intact by respiratory control index. Isolated mitochondria and a 2'-F RNA random library were mixed and the bound RNAs collected. The counter selection was applied with nucleus and unbound RNAs were collected. After 7 rounds of selection, two sequences (Mitomer1 and Mitomer2) were verified to bind to mitochondria and the truncated Mitomer2 (short Mitomer2) showed better binding to isolated mitochondria than Mitomer1.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Mitocôndrias Hepáticas
/
Aptâmeros de Nucleotídeos
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article