Molecular identification of Aedes triseriatus and Aedes hendersoni by a novel duplex polymerase chain reaction assay.
J Am Mosq Control Assoc
; 30(2): 79-82, 2014 Jun.
Article
em En
| MEDLINE
| ID: mdl-25102589
ABSTRACT
Aedes triseriatus is the principal vector of La Crosse virus (LACv), which is the most common cause of pediatric arboviral encephalitis in North America. Here we report a novel species-specific polymerase chain reaction (PCR) assay that differentially identifies Ae. triseriatus and Ae. hendersoni. Because these 2 sibling species differ in their abilities to transmit LACv, accurate identification is critical for surveillance, research, and control programs. This duplex assay can detect the presence of both species in a single PCR reaction and is therefore simpler and faster than previously reported methods.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Reação em Cadeia da Polimerase
/
Aedes
/
Ochlerotatus
/
Insetos Vetores
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Animals
País/Região como assunto:
America do norte
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article