[Cell surface adhesion molecules mediated stretch-induced HL-60 cell adhesion to mouse common carotid arteries].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 30(8): 802-5, 2014 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-25108430
ABSTRACT
OBJECTIVE:
To compare the roles of vascular cell adhesion molecular-1 (VCAM-1) and P-selectin in stretch-induced HL-60 cell adhesion to mouse common carotid arteries.METHODS:
After the isolated mouse common carotid arteries were perfused with HL-60 cells, the unbound leukocytes were washed away and the number of adherent cells in every single field was counted under a microscope. We first observed the effect of stretch on HL-60 cell adhesion to the endothelium of arteries under different magnitudes of luminal hydrostatic pressure. Then, the roles of VCAM-1 and P-selectin in stretch-induced HL-60 cell adhesion to mouse common carotid arteries were compared by the pretreatment of neutralizing antibodies against VCAM-1, P-selectin or isotype-matched control antibody, respectively.RESULTS:
The increase in luminal hydrostatic pressure of carotid arteries led to the increase of the number of HL-60 adhesion cells, suggesting that stretch induced HL-60 cell adhesion in a magnitude-dependent manner. The pretreatment with the isotype-matched control antibody failed to induce a significant change of the number of HL-60 adhesion cells. In contrast, the pretreatment of both VCAM-1 and P-selectin neutralizing antibodies dramatically reduced the number of adherent leukocytes (P<0.05), although no statistical significance was observed between the two groups.CONCLUSION:
Stretch can induce HL-60 cell adhesion to carotid arteries in a magnitude-dependent manner. VCAM-1 and P-selectin play some roles in mechanical stretch-induced HL-60 cell adhesion to mouse common carotid arteries.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Endotélio Vascular
/
Artéria Carótida Primitiva
/
Molécula 1 de Adesão de Célula Vascular
/
Selectina-P
Limite:
Animals
/
Humans
Idioma:
Zh
Ano de publicação:
2014
Tipo de documento:
Article