Type V collagen counteracts osteo-differentiation of human mesenchymal stem cells.
Biologicals
; 42(5): 294-7, 2014 Sep.
Article
em En
| MEDLINE
| ID: mdl-25132375
ABSTRACT
In search of novel gene signatures for osteo-differentiation of mesenchymal stem cells (MSCs), we submitted cDNA preparations from undifferentiated and differentiating MSCs to differential display- and semiquantitative-PCR and found down-regulation of COL5A1 in osteo-induced cultures at days 21 and 28, when the mineralized matrix accumulates. We also cultured osteo-differentiating MSCs onto type V collagen substrates and found a decrease in the accumulation of extracellular calcium compared to those grown in uncoated flasks. To our knowledge, this is first evidence that type V collagen might represent a stromal component that impairs osteogenesis.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteogênese
/
Colágeno Tipo V
/
Células-Tronco Mesenquimais
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article