Nanoscale metal-organic frameworks for real-time intracellular pH sensing in live cells.
J Am Chem Soc
; 136(35): 12253-6, 2014 Sep 03.
Article
em En
| MEDLINE
| ID: mdl-25136764
Real-time measurement of intracellular pH in live cells is of great importance for understanding physiological/pathological processes and developing intracellular drug delivery systems. We report here the first use of nanoscale metal-organic frameworks (NMOFs) for intracellular pH sensing in live cells. Fluorescein isothiocyanate (FITC) was covalently conjugated to a UiO NMOF to afford F-UiO NMOFs with exceptionally high FITC loadings, efficient fluorescence, and excellent ratiometric pH-sensing properties. Upon rapid and efficient endocytosis, F-UiO remained structurally intact inside endosomes. Live cell imaging studies revealed endo- and exocytosis of F-UiO and endosome acidification in real time. Fluorescently labeled NMOFs thus represent a new class of nanosensors for intracellular pH sensing and provide an excellent tool for studying NMOF-cell interactions.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Compostos Organometálicos
/
Fluoresceína-5-Isotiocianato
/
Citoplasma
/
Corantes Fluorescentes
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article