Immunohistochemical toolkit for tracking and quantifying xenotransplanted human stem cells.
Regen Med
; 9(4): 437-52, 2014.
Article
em En
| MEDLINE
| ID: mdl-25159062
ABSTRACT
AIM:
Biomarker-based tracking of human stem cells xenotransplanted into animal models is crucial for studying their fate in the field of cell therapy or tumor xenografting. MATERIALS &METHODS:
Using immunohistochemistry and in situ hybridization, we analyzed the expression of three human-specific biomarkers Ku80, human mitochondria (hMito) and Alu.RESULTS:
We showed that Ku80, hMito and Alu biomarkers are broadly expressed in human tissues with no or low cross-reactivity toward rat, mouse or pig tissues. In vitro, we demonstrated that their expression is stable over time and does not change along the differentiation of human-derived induced pluripotent stem cells or human glial-restricted precursors. We tracked in vivo these cell populations after transplantation in rodent spinal cords using aforementioned biomarkers and human-specific antibodies detecting apoptotic, proliferating or neural-committed cells.CONCLUSION:
This study assesses the human-species specificity of Ku80, hMito and Alu, and proposes useful biomarkers for characterizing human stem cells in xenotransplantation paradigms.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antígenos de Diferenciação
/
Transplante de Células-Tronco
/
Células-Tronco Pluripotentes Induzidas
/
Rastreamento de Células
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article