Protein kinase G regulates ß-synuclein in response to repeated exposure to cocaine in the rat dorsal striatum in a Ca²âº-dependent manner.

Protein kinase G (PKG) activation plays a crucial role in neuronal plasticity after repeated exposure to cocaine in the dorsal striatum. The present study investigated the characteristics of ß-synuclein expression by PKG activation after repeated cocaine administration in the rat dorsal striatum. The results demonstrated that repeated, but not acute, exposure to cocaine (20mg/kg) once a day for 7 consecutive days significantly upregulated expression of ß-synuclein. Furthermore, this upregulation was decreased by the depletion of Ca(2+), but not blockade of Na(+) influx. Blockade of N-methyl-d-aspartate (NMDA) receptors and ryanodine-sensitive Ca(2+) channels also decreased the elevation of ß-synuclein expression by repeated cocaine administration. Inhibition of neuronal nitric oxide synthase, which can activate PKG, did not alter the expression of ß-synuclein elevated by repeated cocaine administration. These findings suggest that the expression of ß-synuclein can be regulated by Ca(2+)-dependent PKG activation via stimulation of NMDA receptors and voltage-operated Ca(2+) channels in the endoplasmic reticulum in the dorsal striatum.