Toward an integrated pipeline for protein biomarker development.
Biochim Biophys Acta
; 1854(6): 677-86, 2015 Jun.
Article
em En
| MEDLINE
| ID: mdl-25218201
ABSTRACT
Protein biomarker development is a multidisciplinary task involving basic, translational and clinical research. Integration of multidisciplinary efforts in a single pipeline is challenging, but crucial to facilitate rational discovery of protein biomarkers and alleviate existing disappointments in the field. In this review, we discuss in detail individual phases of biomarker development pipeline, such as biomarker candidate identification, verification and validation. We focus on mass spectrometry as a principal technique for protein identification and quantification, and discuss complementary -omics approaches for selection of biomarker candidates. Proteomic samples, protein-based clinical laboratory tests and limitations of biomarker development are reviewed in detail, and critical assessment of all phases of biomarker development pipeline is provided. This article is part of a Special Issue entitled Medical Proteomics.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
/
Biomarcadores
/
Proteômica
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article