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Endothelial podosome rosettes regulate vascular branching in tumour angiogenesis.
Seano, Giorgio; Chiaverina, Giulia; Gagliardi, Paolo Armando; di Blasio, Laura; Puliafito, Alberto; Bouvard, Claire; Sessa, Roberto; Tarone, Guido; Sorokin, Lydia; Helley, Dominique; Jain, Rakesh K; Serini, Guido; Bussolino, Federico; Primo, Luca.
Afiliação
  • Seano G; 1] Department of Oncology, University of Torino, Turin 10100, Italy [2] Candiolo Cancer Institute-FPO, IRCCS, Candiolo 10060, Italy [3] Edwin L. Steele Laboratory for Tumor Biology, Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
  • Chiaverina G; 1] Department of Oncology, University of Torino, Turin 10100, Italy [2] Candiolo Cancer Institute-FPO, IRCCS, Candiolo 10060, Italy.
  • Gagliardi PA; 1] Department of Oncology, University of Torino, Turin 10100, Italy [2] Candiolo Cancer Institute-FPO, IRCCS, Candiolo 10060, Italy.
  • di Blasio L; 1] Department of Oncology, University of Torino, Turin 10100, Italy [2] Candiolo Cancer Institute-FPO, IRCCS, Candiolo 10060, Italy.
  • Puliafito A; 1] Department of Oncology, University of Torino, Turin 10100, Italy [2] Candiolo Cancer Institute-FPO, IRCCS, Candiolo 10060, Italy.
  • Bouvard C; UMR-S 765, Université Paris Descartes, Sorbonne Paris Cité, Paris 75006, France.
  • Sessa R; 1] Department of Oncology, University of Torino, Turin 10100, Italy [2] Candiolo Cancer Institute-FPO, IRCCS, Candiolo 10060, Italy.
  • Tarone G; Department of Molecular Biotechnology and Health Sciences, University of Torino, Molecular Biotechnology Center, Turin 10124, Italy.
  • Sorokin L; Institute of Physiological Chemistry and Pathobiochemistry, Muenster University, Muenster 48149, Germany.
  • Helley D; UMR-S 970, Université Paris Descartes, Sorbonne Paris Cité, Paris 75006, France.
  • Jain RK; Edwin L. Steele Laboratory for Tumor Biology, Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
  • Serini G; 1] Department of Oncology, University of Torino, Turin 10100, Italy [2] Candiolo Cancer Institute-FPO, IRCCS, Candiolo 10060, Italy.
  • Bussolino F; 1] Department of Oncology, University of Torino, Turin 10100, Italy [2] Candiolo Cancer Institute-FPO, IRCCS, Candiolo 10060, Italy.
  • Primo L; 1] Department of Oncology, University of Torino, Turin 10100, Italy [2] Candiolo Cancer Institute-FPO, IRCCS, Candiolo 10060, Italy.
Nat Cell Biol ; 16(10): 931-41, 1-8, 2014 Oct.
Article em En | MEDLINE | ID: mdl-25218639
The mechanism by which angiogenic endothelial cells break the physical barrier of the vascular basement membrane and consequently sprout to form new vessels in mature tissues is unclear. Here, we show that the angiogenic endothelium is characterized by the presence of functional podosome rosettes. These extracellular-matrix-degrading and adhesive structures are precursors of de novo branching points and represent a key feature in the formation of new blood vessels. VEGF-A stimulation induces the formation of endothelial podosome rosettes by upregulating integrin α6ß1. In contrast, the binding of α6ß1 integrin to the laminin of the vascular basement membrane impairs the formation of podosome rosettes by restricting α6ß1 integrin to focal adhesions and hampering its translocation to podosomes. Using an ex vivo sprouting angiogenesis assay, transgenic and knockout mouse models and human tumour sample analysis, we provide evidence that endothelial podosome rosettes control blood vessel branching and are critical regulators of pathological angiogenesis.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Estruturas da Membrana Celular / Células Endoteliais / Neoplasias / Neovascularização Patológica Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Estruturas da Membrana Celular / Células Endoteliais / Neoplasias / Neovascularização Patológica Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2014 Tipo de documento: Article