Osteoblast activity of MG-63 cells is enhanced by growth on a lactoferrin-immobilized titanium substrate.
Colloids Surf B Biointerfaces
; 123: 191-8, 2014 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-25277288
ABSTRACT
The aim of this study was to develop a lactoferrin (LF)-immobilized titanium (Ti) substrate to enhance the osteoblast activity of MG-63 cells. Ti substrates were first modified through heparin-dopamine (Hep-DOPA) anchorage. Then, LF was immobilized on the Hep-Ti substrates via electrostatic interactions. Hep-Ti substrates, with or without LF, were evaluated by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle measurements. Sustained release of LF on the Ti substrates was observed over a 28-day period. In vitro studies of osteoblast activity showed increased alkaline phosphatase activity and calcium deposition by MG-63 cells cultured on LF-immobilized Ti substrates as compared to those cultured on pristine Ti substrates, indicating that LF-immobilized Ti substrates were effective at enhancing osteoblast activity.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteoblastos
/
Titânio
/
Lactoferrina
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article