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Critical function of a Chlamydomonas reinhardtii putative polyphosphate polymerase subunit during nutrient deprivation.
Aksoy, Munevver; Pootakham, Wirulda; Grossman, Arthur R.
Afiliação
  • Aksoy M; The Carnegie Institution for Science, Department of Plant Biology, Stanford, California 94305 maksoy1@stanford.edu.
  • Pootakham W; The Carnegie Institution for Science, Department of Plant Biology, Stanford, California 94305 National Center for Genetic Engineering and Biotechnology, Pathum Thani 12120, Thailand.
  • Grossman AR; The Carnegie Institution for Science, Department of Plant Biology, Stanford, California 94305.
Plant Cell ; 26(10): 4214-29, 2014 Oct.
Article em En | MEDLINE | ID: mdl-25281687
Forward genetics was used to isolate Chlamydomonas reinhardtii mutants with altered abilities to acclimate to sulfur (S) deficiency. The ars76 mutant has a deletion that eliminates several genes, including VACUOLAR TRANSPORTER CHAPERONE1 (VTC1), which encodes a component of a polyphosphate polymerase complex. The ars76 mutant cannot accumulate arylsulfatase protein or mRNA and shows marked alterations in levels of many transcripts encoded by genes induced during S deprivation. The mutant also shows little acidocalcisome formation compared with wild-type, S-deprived cells and dies more rapidly than wild-type cells following exposure to S-, phosphorus-, or nitrogen (N)-deficient conditions. Furthermore, the mutant does not accumulate periplasmic L-amino acid oxidase during N deprivation. Introduction of the VTC1 gene specifically complements the ars76 phenotypes, suggesting that normal acidocalcisome formation in cells deprived of S requires VTC1. Our data also indicate that a deficiency in acidocalcisome function impacts trafficking of periplasmic proteins, which can then feed back on the transcription of the genes encoding these proteins. These results and the reported function of vacuoles in degradation processes suggest a major role of the acidocalcisome in reshaping the cell during acclimation to changing environmental conditions.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Polifosfatos / Enxofre / Chlamydomonas reinhardtii Idioma: En Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Polifosfatos / Enxofre / Chlamydomonas reinhardtii Idioma: En Ano de publicação: 2014 Tipo de documento: Article