Quantum dot labelling of adenovirus allows highly sensitive single cell flow and imaging cytometry.
Small
; 11(7): 797-803, 2015 Feb 18.
Article
em En
| MEDLINE
| ID: mdl-25285963
ABSTRACT
A quantum dot method for highly efficient labelling of single adenoviral particles is developed. The technique has no impact on viral fitness and allows the imaging and tracking of virus binding and internalisation events using a variety of techniques including imaging cytometry and confocal microscopy. The method is applied to characterise the tropism of different adenoviral vectors.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Coloração e Rotulagem
/
Adenoviridae
/
Pontos Quânticos
/
Citometria de Fluxo
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article