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Differentiation of human pluripotent stem cells to cells similar to cord-blood endothelial colony-forming cells.
Prasain, Nutan; Lee, Man Ryul; Vemula, Sasidhar; Meador, Jonathan Luke; Yoshimoto, Momoko; Ferkowicz, Michael J; Fett, Alexa; Gupta, Manav; Rapp, Brian M; Saadatzadeh, Mohammad Reza; Ginsberg, Michael; Elemento, Olivier; Lee, Younghee; Voytik-Harbin, Sherry L; Chung, Hyung Min; Hong, Ki Sung; Reid, Emma; O'Neill, Christina L; Medina, Reinhold J; Stitt, Alan W; Murphy, Michael P; Rafii, Shahin; Broxmeyer, Hal E; Yoder, Mervin C.
Afiliação
  • Prasain N; Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana, USA.
  • Lee MR; Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana, USA.
  • Vemula S; Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana, USA.
  • Meador JL; Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana, USA.
  • Yoshimoto M; Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana, USA.
  • Ferkowicz MJ; Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana, USA.
  • Fett A; Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana, USA.
  • Gupta M; Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana, USA.
  • Rapp BM; Department of Surgery, Indiana University School of Medicine, Indianapolis, Indiana, USA.
  • Saadatzadeh MR; Department of Surgery, Indiana University School of Medicine, Indianapolis, Indiana, USA.
  • Ginsberg M; Howard Hughes Medical Institute, Weill Cornell Medical College, New York, New York, USA.
  • Elemento O; Department of Physiology and Biophysics, Weill Cornell Medical College, New York, New York, USA.
  • Lee Y; Department of Medicine, the University of Chicago, Chicago, Illinois, USA.
  • Voytik-Harbin SL; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA.
  • Chung HM; Kon Kuk University School of Medicine, Seoul, South Korea.
  • Hong KS; Stem Cell Research Laboratory, CHA University, Seoul, South Korea.
  • Reid E; Centre for Experimental Medicine, Queen's University Belfast, Belfast, Northern Ireland, UK.
  • O'Neill CL; Centre for Experimental Medicine, Queen's University Belfast, Belfast, Northern Ireland, UK.
  • Medina RJ; Centre for Experimental Medicine, Queen's University Belfast, Belfast, Northern Ireland, UK.
  • Stitt AW; Centre for Experimental Medicine, Queen's University Belfast, Belfast, Northern Ireland, UK.
  • Murphy MP; Department of Surgery, Indiana University School of Medicine, Indianapolis, Indiana, USA.
  • Rafii S; Howard Hughes Medical Institute, Weill Cornell Medical College, New York, New York, USA.
  • Broxmeyer HE; Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana, USA.
  • Yoder MC; Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana, USA.
Nat Biotechnol ; 32(11): 1151-1157, 2014 Nov.
Article em En | MEDLINE | ID: mdl-25306246
ABSTRACT
The ability to differentiate human pluripotent stem cells into endothelial cells with properties of cord-blood endothelial colony-forming cells (CB-ECFCs) may enable the derivation of clinically relevant numbers of highly proliferative blood vessel-forming cells to restore endothelial function in patients with vascular disease. We describe a protocol to convert human induced pluripotent stem cells (hiPSCs) or embryonic stem cells (hESCs) into cells similar to CB-ECFCs at an efficiency of >10(8) ECFCs produced from each starting pluripotent stem cell. The CB-ECFC-like cells display a stable endothelial phenotype with high clonal proliferative potential and the capacity to form human vessels in mice and to repair the ischemic mouse retina and limb, and they lack teratoma formation potential. We identify Neuropilin-1 (NRP-1)-mediated activation of KDR signaling through VEGF165 as a critical mechanism for the emergence and maintenance of CB-ECFC-like cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Diferenciação Celular / Células-Tronco Pluripotentes / Células Endoteliais / Células-Tronco Embrionárias Tipo de estudo: Guideline / Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2014 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Diferenciação Celular / Células-Tronco Pluripotentes / Células Endoteliais / Células-Tronco Embrionárias Tipo de estudo: Guideline / Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2014 Tipo de documento: Article