Interleukin-1ß induces blood-brain barrier disruption by downregulating Sonic hedgehog in astrocytes.
PLoS One
; 9(10): e110024, 2014.
Article
em En
| MEDLINE
| ID: mdl-25313834
ABSTRACT
The blood-brain barrier (BBB) is composed of capillary endothelial cells, pericytes, and perivascular astrocytes, which regulate central nervous system homeostasis. Sonic hedgehog (SHH) released from astrocytes plays an important role in the maintenance of BBB integrity. BBB disruption and microglial activation are common pathological features of various neurologic diseases such as multiple sclerosis, Parkinson's disease, amyotrophic lateral sclerosis, and Alzheimer's disease. Interleukin-1ß (IL-1ß), a major pro-inflammatory cytokine released from activated microglia, increases BBB permeability. Here we show that IL-1ß abolishes the protective effect of astrocytes on BBB integrity by suppressing astrocytic SHH production. Astrocyte conditioned media, SHH, or SHH signal agonist strengthened BBB integrity by upregulating tight junction proteins, whereas SHH signal inhibitor abrogated these effects. Moreover, IL-1ß increased astrocytic production of pro-inflammatory chemokines such as CCL2, CCL20, and CXCL2, which induce immune cell migration and exacerbate BBB disruption and neuroinflammation. Our findings suggest that astrocytic SHH is a potential therapeutic target that could be used to restore disrupted BBB in patients with neurologic diseases.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Barreira Hematoencefálica
/
Astrócitos
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Interleucina-1beta
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Proteínas Hedgehog
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article