A continuous enzyme-coupled assay for triphosphohydrolase activity of HIV-1 restriction factor SAMHD1.
Antimicrob Agents Chemother
; 59(1): 186-92, 2015 Jan.
Article
em En
| MEDLINE
| ID: mdl-25331707
The development of deoxynucleoside triphosphate (dNTP)-based drugs requires a quantitative understanding of any inhibition, activation, or hydrolysis by off-target cellular enzymes. SAMHD1 is a regulatory dNTP-triphosphohydrolase that inhibits HIV-1 replication in human myeloid cells. We describe here an enzyme-coupled assay for quantifying the activation, inhibition, and hydrolysis of dNTPs, nucleotide analogues, and nucleotide analogue inhibitors by triphosphohydrolase enzymes. The assay facilitates mechanistic studies of triphosphohydrolase enzymes and the quantification of off-target effects of nucleotide-based antiviral and chemotherapeutic agents.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bioensaio
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Hidrolases Anidrido Ácido
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Proteínas Monoméricas de Ligação ao GTP
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Avaliação Pré-Clínica de Medicamentos
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article