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Combination of an unbiased amplification method and a resequencing microarray for detecting and genotyping equine arteritis virus.
Hans, Aymeric; Gaudaire, Delphine; Manuguerra, Jean-Claude; Leon, Albertine; Gessain, Antoine; Laugier, Claire; Berthet, Nicolas; Zientara, Stephan.
Afiliação
  • Hans A; ANSES-Dozulé Laboratory for Equine Diseases, Virology Unit, Goustranville, France aymeric.hans@anses.fr.
  • Gaudaire D; ANSES-Dozulé Laboratory for Equine Diseases, Virology Unit, Goustranville, France.
  • Manuguerra JC; Institut Pasteur, Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence (CIBU), Paris, France.
  • Leon A; Frank Duncombe Laboratory, Animal Health Department, IFR146 ICORE, Caen, France.
  • Gessain A; Institut Pasteur, Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, Paris, France Centre National de la Recherche Scientifique, UMR 3569, Paris, France.
  • Laugier C; ANSES-Dozulé Laboratory for Equine Diseases, Virology Unit, Goustranville, France.
  • Berthet N; Institut Pasteur, Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, Paris, France Centre National de la Recherche Scientifique, UMR 3569, Paris, France.
  • Zientara S; Université Paris-Est, Anses Maisons-Alfort Laboratory for Animal Health, UMR1161 Virologie, Maisons-Alfort, France.
J Clin Microbiol ; 53(1): 287-91, 2015 Jan.
Article em En | MEDLINE | ID: mdl-25339390
ABSTRACT
This study shows that an unbiased amplification method applied to equine arteritis virus RNA significantly improves the sensitivity of the real-time reverse transcription-quantitative PCR (RT-qPCR) recommended by the World Organization for Animal Health. Twelve viral RNAs amplified using this method were hybridized on a high-density resequencing microarray for effective viral characterization.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Virologia / Infecções por Arterivirus / Equartevirus / Análise de Sequência com Séries de Oligonucleotídeos / Técnicas de Genotipagem / Doenças dos Cavalos Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Ano de publicação: 2015 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Virologia / Infecções por Arterivirus / Equartevirus / Análise de Sequência com Séries de Oligonucleotídeos / Técnicas de Genotipagem / Doenças dos Cavalos Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Ano de publicação: 2015 Tipo de documento: Article