Podosome-regulating kinesin KIF1C translocates to the cell periphery in a CLASP-dependent manner.
J Cell Sci
; 127(Pt 24): 5179-88, 2014 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-25344256
ABSTRACT
The kinesin KIF1C is known to regulate podosomes, actin-rich adhesion structures that remodel the extracellular matrix during physiological processes. Here, we show that KIF1C is a player in the podosome-inducing signaling cascade. Upon induction of podosome formation by protein kinase C (PKC), KIF1C translocation to the cell periphery intensifies and KIF1C accumulates both in the proximity of peripheral microtubules that show enrichment for the plus-tip-associated proteins CLASPs and around podosomes. Importantly, without CLASPs, both KIF1C trafficking and podosome formation are suppressed. Moreover, chimeric mitochondrially targeted CLASP2 recruits KIF1C, suggesting a transient CLASP-KIF1C association. We propose that CLASPs create preferred microtubule tracks for KIF1C to promote podosome induction downstream of PKC.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cinesinas
/
Extensões da Superfície Celular
/
Proteínas Associadas aos Microtúbulos
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article