A premature termination of human epidermal growth factor receptor transcription in Escherichia coli.
ScientificWorldJournal
; 2014: 830923, 2014.
Article
em En
| MEDLINE
| ID: mdl-25389535
ABSTRACT
Our success in producing an active epidermal growth factor receptor (EGFR) tyrosine kinase in Escherichia coli encouraged us to express the full-length receptor in the same host. Despite its large size, we were successful at producing the full-length EGFR protein fused to glutathione S-transferase (GST) that was detected by Western blot analysis. Moreover, we obtained a majoritarian truncated GST-EGFR form detectable by gel electrophoresis and Western blot. This truncated protein was purified and confirmed by MALDI-TOF/TOF analysis to belong to the N-terminal extracellular region of the EGFR fused to GST. Northern blot analysis showed two transcripts suggesting the occurrence of a transcriptional arrest.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes de Fusão
/
Processamento Alternativo
/
Códon sem Sentido
/
Receptores ErbB
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article