Epithelial rotation promotes the global alignment of contractile actin bundles during Drosophila egg chamber elongation.
Nat Commun
; 5: 5511, 2014 Nov 21.
Article
em En
| MEDLINE
| ID: mdl-25413675
ABSTRACT
Tissues use numerous mechanisms to change shape during development. The Drosophila egg chamber is an organ-like structure that elongates to form an elliptical egg. During elongation the follicular epithelial cells undergo a collective migration that causes the egg chamber to rotate within its surrounding basement membrane. Rotation coincides with the formation of a 'molecular corset', in which actin bundles in the epithelium and fibrils in the basement membrane are all aligned perpendicular to the elongation axis. Here we show that rotation plays a critical role in building the actin-based component of the corset. Rotation begins shortly after egg chamber formation and requires lamellipodial protrusions at each follicle cell's leading edge. During early stages, rotation is necessary for tissue-level actin bundle alignment, but it becomes dispensable after the basement membrane is polarized. This work highlights how collective cell migration can be used to build a polarized tissue organization for organ morphogenesis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oogênese
/
Óvulo
/
Pseudópodes
/
Proteínas Contráteis
/
Drosophila melanogaster
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article