Ultrasonication-assisted one-step self-assembly preparation of biocompatible fluorescent-magnetic nanobeads for rare cancer cell detection.
Nanotechnology
; 25(50): 505603, 2014 Dec 19.
Article
em En
| MEDLINE
| ID: mdl-25426596
ABSTRACT
Multifunctional nanomaterials simultaneously possessing attractive properties, such as strong fluorescent intensity, excellent superparamagnetic behavior, easy modification and good biocompatibility, are always desired in a wide range of applications. In this work, we present a facile ultrasonication-assisted one-step self-assembly strategy for the fabrication of smart fluorescent-magnetic nanobeads (FMNBs) without using a matrix. Via one-step ultrasonication, organic-soluble superparamagnetic nanoparticles (MNPs) and quantum dots (QDs) were automatically encapsulated by amphiphilic (2-hydroxyl-3-dodecanoxyl) propylcarboxymethylchitosans (HDP-CMCHSs) through hydrophobic interaction to form hydrophilic FMNBs, presenting a good QD fluorescent property and a strong MNP magnetic response. The outer surface of the FMNBs was derived from natural biopolymer chitosans, enabling FMNBs with good biocompatibility and convenience for biological modification. As-prepared FMNBs can be easily modified with streptavidin, facilitating bioconjugation with biotin-labeled human epidermal growth factor (hEGF). hEGF-functionalized FMNBs are able to specifically recognize and capture rare target cells spiked in white blood cells, and the recovered cells can be further cultured for a long time. All of these excellent properties make nanobeads promising for circulating tumor cell (CTC) detection.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sonicação
/
Pontos Quânticos
/
Nanopartículas de Magnetita
/
Células Neoplásicas Circulantes
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article