Long non-coding RNA-mediated transcriptional interference of a permease gene confers drug tolerance in fission yeast.
Nat Commun
; 5: 5576, 2014 Nov 27.
Article
em En
| MEDLINE
| ID: mdl-25428589
ABSTRACT
Most long non-coding RNAs (lncRNAs) encoded by eukaryotic genomes remain uncharacterized. Here we focus on a set of intergenic lncRNAs in fission yeast. Deleting one of these lncRNAs exhibited a clear phenotype drug sensitivity. Detailed analyses of the affected locus revealed that transcription of the nc-tgp1 lncRNA regulates drug tolerance by repressing the adjacent phosphate-responsive permease gene transporter for glycerophosphodiester 1 (tgp1(+)). We demonstrate that the act of transcribing nc-tgp1 over the tgp1(+) promoter increases nucleosome density, prevents transcription factor access and thus represses tgp1(+) without the need for RNA interference or heterochromatin components. We therefore conclude that tgp1(+) is regulated by transcriptional interference. Accordingly, decreased nc-tgp1 transcription permits tgp1(+) expression upon phosphate starvation. Furthermore, nc-tgp1 loss induces tgp1(+) even in repressive conditions. Notably, drug sensitivity results directly from tgp1(+) expression in the absence of the nc-tgp1 RNA. Thus, transcription of an lncRNA governs drug tolerance in fission yeast.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
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Schizosaccharomyces
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RNA Fúngico
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Proteínas de Schizosaccharomyces pombe
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Farmacorresistência Fúngica
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Interferência de RNA
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RNA Longo não Codificante
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Antifúngicos
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article