Fetal calf sera can distort cell-based luminescent proteasome assays through heat-resistant chymotrypsin-like activity.

Dettmer, Susan; Theile, Dirk; Seckinger, Anja; Burhenne, Jürgen; Weiss, Johanna

Dettmer, Susan; Theile, Dirk; Seckinger, Anja; Burhenne, Jürgen; Weiss, Johanna.

Afiliação

Dettmer S; Department of Clinical Pharmacology and Pharmacoepidemiology, Heidelberg University Hospital, D-69120 Heidelberg, Germany.
Theile D; Department of Clinical Pharmacology and Pharmacoepidemiology, Heidelberg University Hospital, D-69120 Heidelberg, Germany.
Seckinger A; Department of Internal Medicine V, Oncology, Hematology, and Rheumatology, Heidelberg University Hospital, D-69120 Heidelberg, Germany.
Burhenne J; Department of Clinical Pharmacology and Pharmacoepidemiology, Heidelberg University Hospital, D-69120 Heidelberg, Germany.
Weiss J; Department of Clinical Pharmacology and Pharmacoepidemiology, Heidelberg University Hospital, D-69120 Heidelberg, Germany. Electronic address: johanna.weiss@med.uni-heidelberg.de.

Anal Biochem ; 471: 23-5, 2015 Feb 15.

Article em En | MEDLINE | ID: mdl-25447494

Assuntos

Artefatos; Quimotripsina/metabolismo; Ensaios Enzimáticos/métodos; Medições Luminescentes; Complexo de Endopeptidases do Proteassoma/metabolismo; Soro/metabolismo; Animais; Bovinos

Palavras-chave

Bortezomib; Carfilzomib; Fetal calf serum; Heat resistance; Proteasome activity; Proteasome-Glo chymotrypsin-like cell-based assays

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quimotripsina / Artefatos / Soro / Complexo de Endopeptidases do Proteassoma / Ensaios Enzimáticos / Medições Luminescentes Limite: Animals Idioma: En Ano de publicação: 2015 Tipo de documento: Article

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