Effective modulation of transforming growth factor-ß1 expression through engineered microRNA-based plasmid-loaded nanospheres.
Cytotherapy
; 17(3): 320-9, 2015 Mar.
Article
em En
| MEDLINE
| ID: mdl-25457276
BACKGROUND AIMS: Sustained gene delivery could be particularly necessary in modulating gene expression in healing intrasynovial tendons and may be a promising approach for preventing adhesions. METHODS: Polylactic-co-glycolic acid nanospheres were prepared and were then incorporated with plasmids expressing enhanced green fluorescence protein and miRNA for inhibiting the transforming growth factor-ß1 gene expression. RESULTS: We demonstrated that cultured tenocytes could be effectively transfected by means of nanosphere/plasmid. The expression of transforming growth factor-ß1 is significantly downregulated in healing chicken flexor tendon treated with nanosphere/plasmid. Histology analysis did not demonstrate any significant inflammation or necrosis in tendons injected with nanosphere/plasmid after surgery. CONCLUSIONS: Nanosphere/plasmid may be a promising non-viral vector for gene therapy of the injured flexor tendon.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
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Traumatismos dos Tendões
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Cicatrização
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Terapia Genética
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MicroRNAs
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Fator de Crescimento Transformador beta1
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Nanosferas
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article