[Molecular identification of 15 species of Ilex genus based on ITS sequence analysis ].

ABSTRACT

OBJECTIVE: To establish molecular method for identification of Ilex plants using the internal transcribed spacer(ITS) bar- code. METHODS: DNA was extracted using centrifugal columnar kits. The ITS sequences of all experimental samples were amplified and sequenced using a pair of universal primers. The alignment and the phylogenetic tree were performed in MEGA5. 0. RESULTS: The length of ITS sequence in each species was 759 bp,in which there were 216 sites discrepancy. CONCLUSION: DNA barcoding technology based on ITS sequence can be used to identify plants of Ilex genus.