Comparison of three protein A-gold immune electron microscopy methods for detecting rotaviruses.
J Virol Methods
; 25(1): 109-18, 1989 Jul.
Article
em En
| MEDLINE
| ID: mdl-2550499
ABSTRACT
Three immune electron microscopic (IEM) methods employing protein A-colloidal gold (PAG) were compared with direct electron microscopy (DEM) and direct IEM (DIEM) techniques for their abilities to detect simian rotavirus (SA11) and human rotavirus. Using PAG IEM the number of rotavirus particles per grid square was 10 to 50 times and 2 to 5 times higher than observed with DEM and DIEM, respectively. A comparison of sensitivities among the three PAG IEM methods showed that the trapping efficiency for SA11 and human rotavirus was significantly enhanced by a serum-in-agar (SIA) method. The SIA method appeared to be a more reliable and sensitive alternative for detection and quantitation of viral particles.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imuno-Histoquímica
/
Rotavirus
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1989
Tipo de documento:
Article