Silencing of antibiotic resistance in E. coli with engineered phage bearing small regulatory RNAs.
ACS Synth Biol
; 3(12): 1003-6, 2014 Dec 19.
Article
em En
| MEDLINE
| ID: mdl-25524110
ABSTRACT
In response to emergent antibiotic resistance, new strategies are needed to enhance the effectiveness of existing antibiotics. Here, we describe a phagemid-delivered, RNA-mediated system capable of directly knocking down antibiotic resistance phenotypes. Small regulatory RNAs (sRNAs) were designed to specifically inhibit translation of chloramphenicol acetyltransferase and kanamycin phosphotransferase. Nonlytic phagemids coding for sRNA expression were able to infect and restore chloramphenicol and kanamycin sensitivity to populations of otherwise resistant E. coli. This modular system could easily be extended to other bacteria with resistance profiles that depend on specific transcripts.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bacteriófagos
/
Resistência Microbiana a Medicamentos
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RNA Viral
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Inativação Gênica
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Escherichia coli
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article