Characterization and homologous overexpression of an N-acetylglucosaminidase Nag1 from Trichoderma reesei.
Biochem Biophys Res Commun
; 459(2): 184-188, 2015 Apr 03.
Article
em En
| MEDLINE
| ID: mdl-25534854
ABSTRACT
Trichoderma reesei is thought to be a promising recombinant host for the production and secretion of complex proteins due to its ability to secrete large amounts of proteins. In this study we identified a functional N-acetyl-ß-glucosaminidase (NAGase) gene Nag1 in T. reesei. Nag1, a putative gene encoding a GH 20 family NAGase in T. reesei, was cloned and homologous overexpressed in the T. reesei RutC30ΔU3 with a strong cellobiohydrolase1 gene (cbh1) promoter. Nag1 was secreted in its active form and the highest expression level was around 499.85IU/ml. Nag1 has a molecular mass of 80kDa. The optimum pH and temperature were 4.0 and 60°C, respectively.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Acetilglucosaminidase
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Trichoderma
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Proteínas Fúngicas
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article