Effect of bone morphogenetic protein 7 on differentiation of adipose derived mesenchymal stem cells into brown adipocytes in rats.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
; 36(6): 654-9, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-25556742
ABSTRACT
OBJECTIVE:
To evaluate the effect of bone morphogenetic protein(BMP7)on the differentiation of adipose derived mesenchymal stem cells(AD-MSCs)isolated from different adipose tissues into brown adipocytes in rats.METHODS:
Primary AD-MSCs were isolated from rate interscapular brown adipose tissue(iBAT),inguinal subcutaneous white adipose tissue(sWAT),and epididymal white adipose tissue(eWAT),respectively,and then cultivated in vitro. Differentiation of AD-MSCs into brown adipocytes was induced by BMP7. The characteristics of brown adipocytes were detected by immunofluorescence staining and oil red staining of cells. The expression levels of brown adipocyte-related genes were detected by polymerase chain reaction.RESULTS:
AD-MSCs from iBAT and sWAT were differentiated into cluster multilocular cells,which were stained red by oil red "O"staining and showed uncoupling protein 1-positive by immunofluorescent staining method. AD-MSCs from eWAT had a small number of scattered multilocular cells and showed uncoupling protein 1-negative. The results of reverse transcription-polymerase chain reaction showed that the uncoupling protein 1 gene was highly expressed in the iBAT group and sWAT group but was negative in the eWAT group.CONCLUSION:
AD-MSCs isolated from different adipose tissues in rats have different gene expression profiles and differentiation potentials.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tecido Adiposo Marrom
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Diferenciação Celular
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Adipócitos Marrons
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Proteína Morfogenética Óssea 7
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Células-Tronco Mesenquimais
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article