The RNA sensor RIG-I dually functions as an innate sensor and direct antiviral factor for hepatitis B virus.
Immunity
; 42(1): 123-32, 2015 Jan 20.
Article
em En
| MEDLINE
| ID: mdl-25557055
ABSTRACT
Host innate recognition triggers key immune responses for viral elimination. The sensing mechanism of hepatitis B virus (HBV), a DNA virus, and the subsequent downstream signaling events remain to be fully clarified. Here we found that type III but not type I interferons are predominantly induced in human primary hepatocytes in response to HBV infection, through retinoic acid-inducible gene-I (RIG-I)-mediated sensing of the 5'-ε region of HBV pregenomic RNA. In addition, RIG-I could also counteract the interaction of HBV polymerase (P protein) with the 5'-ε region in an RNA-binding dependent manner, which consistently suppressed viral replication. Liposome-mediated delivery and vector-based expression of this ε region-derived RNA in liver abolished the HBV replication in human hepatocyte-chimeric mice. These findings identify an innate-recognition mechanism by which RIG-I dually functions as an HBV sensor activating innate signaling and to counteract viral polymerase in human hepatocytes.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Viral
/
Produtos do Gene pol
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Vírus da Hepatite B
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Hepatite B Crônica
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Hepatócitos
/
Fígado
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Proteínas de Membrana
/
Proteínas do Tecido Nervoso
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Child, preschool
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Female
/
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article