The Potyviridae P1a leader protease contributes to host range specificity.
Virology
; 476: 264-270, 2015 Feb.
Article
em En
| MEDLINE
| ID: mdl-25562450
ABSTRACT
The P1a protein of the ipomovirus Cucumber vein yellowing virus is one of the self-cleavage serine proteases present in Potyviridae family members. P1a is located at the N-terminal end of the viral polyprotein, and is closely related to potyviral P1 protease. For its proteolytic activity, P1a requires a still unknown host factor; this might be linked to involvement in host specificity. Here we built a series of constructs and chimeric viruses to help elucidate the role of P1a cleavage in host range definition. We demonstrate that host-dependent separation of P1a from the remainder of the polyprotein is essential for suppressing RNA silencing defenses and for efficient viral infection. These findings support the role of viral proteases as important determinants in host adaptation.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças das Plantas
/
Proteínas Virais
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Potyviridae
/
Serina Proteases
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Especificidade de Hospedeiro
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article