[Research methods in protein-protein and protein-nucleic acid interactions and application in the study of human enterovirus A71].
Bing Du Xue Bao
; 30(5): 587-93, 2014 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-25562971
ABSTRACT
Methods for analyses of protein-protein interactions include yeast two hybrid (Y2H), phage dis- play (PD), co-immunoprecipitation (Co-IP), glutathione S-transferase pull-down (GST pull-down), cellular co-localization, far-western blotting, virus overlay protein binding assay (VOPBA), surface plasmon resonance (SPR), and fluorescence resonance energy transfer (FRET). Technologies for the detection of protein-nucleic acid interactions include yeast one hybrid (Y1H), chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assay (EMSA), Southwestern blotting, reporter gene, Co-IP, GST pull-down, and PD. These methods are often used in the study of the human enterovirus A71 (EV-A71) by our research team. Reviews in the Chinese literature in this field are lacking, so we reviewed applications of these methods in the study of EV-A71. This review may impart important knowledge in the research of other viruses with regard to protein-protein and protein-nucleic acid interactions.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
RNA Viral
/
Enterovirus Humano A
Idioma:
Zh
Ano de publicação:
2014
Tipo de documento:
Article