In vivo cross-linking followed by polyA enrichment to identify yeast mRNA binding proteins.
Methods Mol Biol
; 1259: 35-47, 2015.
Article
em En
| MEDLINE
| ID: mdl-25579578
ABSTRACT
mRNA binding proteins regulate gene expression by controlling the processing, localization, decay, and translation of messenger RNAs (mRNAs). To fully understand this process, it is necessary to identify the complete set of mRNA binding proteins. This work describes a method for the systematic identification of yeast mRNA binding proteins. This method applies in vivo UV cross-linking, affinity pull-down of polyA(+) mRNAs, and analysis by mass spectrometry to identify proteins that directly bind to mRNAs.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poli A
/
RNA Mensageiro
/
Proteínas de Ligação a RNA
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article