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Improved and expanded Q-system reagents for genetic manipulations.
Riabinina, Olena; Luginbuhl, David; Marr, Elizabeth; Liu, Sha; Wu, Mark N; Luo, Liqun; Potter, Christopher J.
Afiliação
  • Riabinina O; The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
  • Luginbuhl D; 1] Howard Hughes Medical Institute, Stanford University, Stanford, California, USA. [2] Department of Biology, Stanford University, Stanford, California, USA.
  • Marr E; The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
  • Liu S; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
  • Wu MN; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
  • Luo L; 1] Howard Hughes Medical Institute, Stanford University, Stanford, California, USA. [2] Department of Biology, Stanford University, Stanford, California, USA.
  • Potter CJ; The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Nat Methods ; 12(3): 219-22, 5 p following 222, 2015 Mar.
Article em En | MEDLINE | ID: mdl-25581800
ABSTRACT
The Q system is a repressible binary expression system for transgenic manipulations in living organisms. Through protein engineering and in vivo functional tests, we report here variants of the Q-system transcriptional activator, including QF2, for driving strong and ubiquitous expression in all Drosophila tissues. Our QF2, Gal4QF and LexAQF chimeric transcriptional activators substantially enrich the toolkit available for transgenic regulation in Drosophila melanogaster.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Engenharia Genética / Transativadores / Transgenes / Drosophila melanogaster Limite: Animals Idioma: En Ano de publicação: 2015 Tipo de documento: Article
Texto completo
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PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Engenharia Genética / Transativadores / Transgenes / Drosophila melanogaster Limite: Animals Idioma: En Ano de publicação: 2015 Tipo de documento: Article