Your browser doesn't support javascript.
loading
Determination and pharmacokinetic study of pirfenidone in rat plasma by UPLC-MS/MS.
Sun, Wei; Jiang, Zhe-li; Zhou, Lei; Chen, Rui-min; Wang, Zhe; Li, Wan-shu; Jiang, Shuo-min; Hu, Guo-xin; Chen, Rui-jie.
Afiliação
  • Sun W; The Second Affiliated Hospital & Yuying Children' s Hospital of Wenzhou Medical University, Wenzhou 325027, China.
  • Jiang ZL; School of Pharmacy, Wenzhou Medical University, Wenzhou 325035, China.
  • Zhou L; The Second Affiliated Hospital & Yuying Children' s Hospital of Wenzhou Medical University, Wenzhou 325027, China.
  • Chen RM; The Second Affiliated Hospital & Yuying Children' s Hospital of Wenzhou Medical University, Wenzhou 325027, China.
  • Wang Z; The Second Affiliated Hospital & Yuying Children' s Hospital of Wenzhou Medical University, Wenzhou 325027, China.
  • Li WS; Ningbo Municipal Hospital of TCM, Ningbo 315010, China.
  • Jiang SM; The Second Affiliated Hospital & Yuying Children' s Hospital of Wenzhou Medical University, Wenzhou 325027, China.
  • Hu GX; School of Pharmacy, Wenzhou Medical University, Wenzhou 325035, China.
  • Chen RJ; The Second Affiliated Hospital & Yuying Children' s Hospital of Wenzhou Medical University, Wenzhou 325027, China. Electronic address: crj@wzhealth.com.
Article em En | MEDLINE | ID: mdl-25596380
A rapid, sensitive and selective ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed and validated for the determination and pharmacokinetic investigation of pirfenidone in rat plasma. Sample preparation was accomplished through a simple one-step deproteinization procedure with 0.2 mL of acetonitrile to a 0.1 mL plasma sample. Plasma samples were separated by UPLC on an Acquity UPLC BEH C18 column using a mobile phase consisting of acetonitrile-0.1% formic acid in water with gradient elution. The total run time was 3.0 min and the elution of pirfenidone was at 1.39 min. The detection was performed on a triple quadrupole tandem mass spectrometer in the multiple reaction-monitoring (MRM) mode using the respective transitions m/z 186.2→92.1 for pirfenidone and m/z 237.1→194.2 for carbamazepine (IS), respectively. The calibration curve was linear over the range of 5-2000 ng/mL with a lower limit of quantitation (LLOQ) of 5 ng/mL. Mean recovery of pirfenidone in plasma was in the range of 80.4-84.3%. Intra-day and inter-day precision were both <12.1%. This method was successfully applied in pharmacokinetic study after oral administration of 10.0mg/kg pirfenidone in rats.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Piridonas / Cromatografia Líquida de Alta Pressão / Espectrometria de Massas em Tandem Limite: Animals Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Piridonas / Cromatografia Líquida de Alta Pressão / Espectrometria de Massas em Tandem Limite: Animals Idioma: En Ano de publicação: 2015 Tipo de documento: Article