Controlled assembly of artificial protein-protein complexes via DNA duplex formation.
Bioconjug Chem
; 26(3): 427-34, 2015 Mar 18.
Article
em En
| MEDLINE
| ID: mdl-25603321
ABSTRACT
DNA-protein conjugates have found a wide range of applications. This study demonstrates the formation of defined, non-native protein-protein complexes via the site specific labeling of two proteins of interest with complementary strands of single-stranded DNA in vitro. This study demonstrates that the affinity of two DNA-protein conjugates for one another may be tuned by the use of variable lengths of DNA allowing reversible control of complex formation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
DNA
/
DNA de Cadeia Simples
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Proteínas de Fluorescência Verde
/
Domínios e Motivos de Interação entre Proteínas
/
Proteínas Luminescentes
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article