Live-cell assessment of mitochondrial reactive oxygen species using dihydroethidine.
Methods Mol Biol
; 1264: 161-9, 2015.
Article
em En
| MEDLINE
| ID: mdl-25631012
ABSTRACT
Reactive oxygen species (ROS) play an important role in both physiology and pathology. Mitochondria are an important source of the primary ROS superoxide. However, accurate detection of mitochondrial superoxide especially in living cells remains a difficult task. Here, we describe a method and the pitfalls to detect superoxide in both mitochondria and the entire cell using dihydroethidium (HEt) and live-cell microscopy.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dicarbetoxi-Di-Hidrocolidina
/
Espécies Reativas de Oxigênio
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Microscopia
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Mitocôndrias
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article