Absence of giant blood Marseille-like virus DNA detection by polymerase chain reaction in plasma from healthy US blood donors and serum from multiply transfused patients from Cameroon.
Transfusion
; 55(6): 1256-62, 2015 Jun.
Article
em En
| MEDLINE
| ID: mdl-25645088
ABSTRACT
BACKGROUND:
A new Marseilleviridae virus family member, giant blood Marseille-like (GBM) virus, was recently reported in persons from France in the serum of an infant with adenitis, in the blood of 4% of healthy blood donors, and in 9% of multiply transfused thalassemia patients. These results suggested the presence of a nucleocytoplasmic large DNA virus potentially transmissible by blood product transfusion. STUDY DESIGN ANDMETHODS:
To investigate this possibility we tested the plasma from 113 US blood donors and 74 multiply transfused Cameroon patients for GBM viral DNA using highly sensitive polymerase chain reaction (PCR) assays.RESULTS:
GBM DNA was not detected by nested PCR in any of these 187 human specimens.CONCLUSIONS:
Further testing is required to confirm the occurrence of human GBM virus infections.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Viremia
/
Doadores de Sangue
/
DNA Viral
/
Reação em Cadeia da Polimerase
/
Infecções por Vírus de DNA
/
Vírus de DNA
Tipo de estudo:
Diagnostic_studies
Limite:
Adult
/
Humans
/
Male
/
Middle aged
País/Região como assunto:
Africa
/
America do norte
/
Europa
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article