A correlation study of telomere length in peripheral blood leukocytes and kidney function with age.

ABSTRACT

The current study aimed to investigate the association between telomere length in peripheral blood leukocytes and kidney function in various age groups of a healthy population. A total of 139 healthy individuals were divided into five groups according to their age 35­44, 45­54, 55­64, 65­74 and >75 years old. Peripheral blood leukocytes were obtained and the telomere restriction fragment (TRF) length was assayed using a digoxigenin­labeled hybridization probe in Southern blot assays. Laboratory assays of kidney function were also performed. A correlation was observed between TRF length and age (r=­0.314, P<0.001), with the telomere length of the individuals >75 years group being significantly shorter than the telomere length of the 35­44, 45­54 and 55­64 years age groups (P<0.05). By contrast, the TRF length for males versus females did not differ for any of the age groups, while a correlation was observed between TRF length and serum levels of cystatin C (r=­0.195, P<0.05). There was also a correlation between TRF length and glomerular filtration rate (r=­0.184, P<0.05). The current study demonstrated that in this cohort, leukocyte telomere length reduced with age and was correlated with serum levels of cystatin C and glomerular filtration rate. Therefore, TRF length is associated with kidney function and may serve as a marker of aging.