The biological basis for poly-L-lactic acid-induced augmentation.
J Dermatol Sci
; 78(1): 26-33, 2015 Apr.
Article
em En
| MEDLINE
| ID: mdl-25703057
ABSTRACT
BACKGROUND:
Granulomatous reactions to poly-L-lactic acid (PLLA)-based filler have been described previously. Neither the biological background of these partly late-onset reactions or the desired augmenting effect of PLLA has been studied to date. Histological studies have revealed foreign body reactions and foreign body giant cell formation.OBJECTIVE:
The aim of this study was to increase our knowledge about the biological mechanisms behind the augmenting effect of PLLA-based filler.METHODS:
We characterised the cell infiltrate and collagen type of PLLA-treated tissue by immunofluorescence staining. The expression of genes related to collagen metabolism was determined.RESULTS:
CD68(+) macrophages were found next to PLLA. CD90(+) fibroblasts were found alongside. αSMA-positive structures indicated myofibroblasts and neovascularisation. Substantial collagen type III deposition was detected next to PLLA particles and collagen type I was found at the periphery of PLLA encapsulations. mRNA expression for collagen type I and III transcripts, as well as for TGFß1 and TIMP1, was upregulated significantly.CONCLUSION:
PLLA-induced augmentation is most likely based on capsule formation orchestrating macrophages, (myo-)fibroblasts, and collagen type I and III fibres. We observed considerably slower degradation of PLLA particles than described previously. Thus PLLA particles were still retrievable 28 months after subcutaneous application.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polímeros
/
Técnicas Cosméticas
/
Ácido Láctico
/
Tela Subcutânea
/
Fibroblastos
/
Preenchedores Dérmicos
/
Macrófagos
Tipo de estudo:
Clinical_trials
/
Observational_studies
/
Risk_factors_studies
País/Região como assunto:
Europa
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article