Over-expression of miR-125a-5p inhibits proliferation in C2C12 myoblasts by targeting E2F3.
Acta Biochim Biophys Sin (Shanghai)
; 47(4): 244-9, 2015 Apr.
Article
em En
| MEDLINE
| ID: mdl-25733534
ABSTRACT
MicroRNAs (miRNAs) are a class of small non-coding RNAs of 20-25 nucleotides in length. It has been shown that miRNAs play important roles in the proliferation of many types of cells, including myoblasts. In this study, we used real-time quantitative polymerase chain reaction, western blotting, EdU, flow cytometry, and CCK-8 assay to explore the role of miR-125a-5p during the proliferation of C2C12 myoblasts. It was found that the expression of miR-125a-5p was decreased during C2C12 myoblast proliferation. Over-expression of miR-125a-5p inhibited C2C12 myoblast proliferation as indicated by EdU staining, flow cytometry, and CCK8 assay. It was also found that miR-125a-5p could negatively regulate E2F3 expression at posttranscriptional level, via a specific target site in the 3' untranslated region. Knockdown of E2F3 showed a similar inhibitory effect on C2C12 myoblast proliferation. Thus, our findings suggest that miR-125a-5p may act as a negative regulator of C2C12 myoblast proliferation by targeting E2F3.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Expressão Gênica
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Mioblastos
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MicroRNAs
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Proliferação de Células
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Fator de Transcrição E2F3
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article