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Efficiently editing the vaccinia virus genome by using the CRISPR-Cas9 system.
Yuan, Ming; Zhang, Wensheng; Wang, Jun; Al Yaghchi, Chadwan; Ahmed, Jahangir; Chard, Louisa; Lemoine, Nick R; Wang, Yaohe.
Afiliação
  • Yuan M; Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, United Kingdom.
  • Zhang W; Cambridge Suda Genome Research Center, Soochow University, Soochow, Jiangsu Province, People's Republic of China Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.
  • Wang J; Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, United Kingdom.
  • Al Yaghchi C; Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, United Kingdom.
  • Ahmed J; Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, United Kingdom.
  • Chard L; Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, United Kingdom.
  • Lemoine NR; Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, United Kingdom Sino-British Research Centre for Molecular Oncology, Zhengzhou University, China bci-director@qmul.ac.uk Yaohe.wang@qmul.ac.uk.
  • Wang Y; Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, United Kingdom Sino-British Research Centre for Molecular Oncology, Zhengzhou University, China bci-director@qmul.ac.uk Yaohe.wang@qmul.ac.uk.
J Virol ; 89(9): 5176-9, 2015 May.
Article em En | MEDLINE | ID: mdl-25741005
ABSTRACT
Vaccinia virus (VACV) continues to be used in immunotherapy for the prevention of infectious diseases and treatment of cancer since its use for the eradication of smallpox. However, the current method of editing the VACV genome is not efficient. Here, we demonstrate that the CRISPR-Cas9 system can be used to edit the VACV genome rapidly and efficiently. Additionally, a set of 8,964 computationally designed unique guide RNAs (gRNAs) targeting all VACV genes will be valuable for the study of VACV gene functions.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Recombinação Genética / Vaccinia virus / Genoma Viral / Sistemas CRISPR-Cas / Biologia Molecular Idioma: En Ano de publicação: 2015 Tipo de documento: Article
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Recombinação Genética / Vaccinia virus / Genoma Viral / Sistemas CRISPR-Cas / Biologia Molecular Idioma: En Ano de publicação: 2015 Tipo de documento: Article