Efficiently editing the vaccinia virus genome by using the CRISPR-Cas9 system.
J Virol
; 89(9): 5176-9, 2015 May.
Article
em En
| MEDLINE
| ID: mdl-25741005
ABSTRACT
Vaccinia virus (VACV) continues to be used in immunotherapy for the prevention of infectious diseases and treatment of cancer since its use for the eradication of smallpox. However, the current method of editing the VACV genome is not efficient. Here, we demonstrate that the CRISPR-Cas9 system can be used to edit the VACV genome rapidly and efficiently. Additionally, a set of 8,964 computationally designed unique guide RNAs (gRNAs) targeting all VACV genes will be valuable for the study of VACV gene functions.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Recombinação Genética
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Vaccinia virus
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Genoma Viral
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Sistemas CRISPR-Cas
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Biologia Molecular
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article