Tissue-specific gene expression profiling by cell sorting.
Methods Mol Biol
; 1284: 175-83, 2015.
Article
em En
| MEDLINE
| ID: mdl-25757772
ABSTRACT
Many cell populations have been labeled using stable reporters containing a fluorescent protein. These same marker lines can be used to capture specific cell types or marked cell populations after a brief enzymatic digestion to dissociate tissues. Here, we describe the use of Fluorescence Activated Cell Sorting (FACS) to isolate a rare population of cells marked with Green Fluorescent Protein (GFP). The FACS is a powerful selection tool that can be used to isolate high-quality RNA while selecting for fluorescence, cell size, and other properties.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regulação da Expressão Gênica de Plantas
/
Perfilação da Expressão Gênica
/
Citometria de Fluxo
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article