Visualizing transient Watson-Crick-like mispairs in DNA and RNA duplexes.
Nature
; 519(7543): 315-20, 2015 Mar 19.
Article
em En
| MEDLINE
| ID: mdl-25762137
ABSTRACT
Rare tautomeric and anionic nucleobases are believed to have fundamental biological roles, but their prevalence and functional importance has remained elusive because they exist transiently, in low abundance, and involve subtle movements of protons that are difficult to visualize. Using NMR relaxation dispersion, we show here that wobble dGâ¢dT and rGâ¢rU mispairs in DNA and RNA duplexes exist in dynamic equilibrium with short-lived, low-populated Watson-Crick-like mispairs that are stabilized by rare enolic or anionic bases. These mispairs can evade Watson-Crick fidelity checkpoints and form with probabilities (10(-3) to 10(-5)) that strongly imply a universal role in replication and translation errors. Our results indicate that rare tautomeric and anionic bases are widespread in nucleic acids, expanding their structural and functional complexity beyond that attainable with canonical bases.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
RNA
/
Pareamento de Bases
/
Ácidos Nucleicos Heteroduplexes
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article