A novel technique for detecting antibiotic-resistant typhoid from rapid diagnostic tests.

Nic Fhogartaigh, Caoimhe; Dance, David A B; Davong, Viengmon; Tann, Pisey; Phetsouvanh, Rattanaphone; Turner, Paul; Dittrich, Sabine; Newton, Paul N

Nic Fhogartaigh, Caoimhe; Dance, David A B; Davong, Viengmon; Tann, Pisey; Phetsouvanh, Rattanaphone; Turner, Paul; Dittrich, Sabine; Newton, Paul N.

Afiliação

Nic Fhogartaigh C; Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Vientiane, Lao PDR Public Health England, London, England, United Kingdom.
Dance DA; Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Vientiane, Lao PDR Public Health England, London, England, United Kingdom Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, Old Road Campus, University of Oxford, Oxford, England.
Davong V; Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Vientiane, Lao PDR.
Tann P; Cambodia-Oxford Medical Research Unit, Angkor Hospital for Children, Siem Reap, Cambodia.
Phetsouvanh R; Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Vientiane, Lao PDR.
Turner P; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, Old Road Campus, University of Oxford, Oxford, England Cambodia-Oxford Medical Research Unit, Angkor Hospital for Children, Siem Reap, Cambodia.
Dittrich S; Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Vientiane, Lao PDR Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, Old Road Campus, University of Oxford, Oxford, England sabine@tropmedres.ac.
Newton PN; Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Vientiane, Lao PDR Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, Old Road Campus, University of Oxford, Oxford, England.

J Clin Microbiol ; 53(5): 1758-60, 2015 May.

Article em En | MEDLINE | ID: mdl-25762768

ABSTRACT

ABSTRACT

Fluoroquinolone-resistant typhoid is increasing. An antigen-detecting rapid diagnostic test (RDT) can rapidly diagnose typhoid from blood cultures. A simple, inexpensive molecular technique performed with DNA from positive RDTs accurately identified gyrA mutations consistent with phenotypic susceptibility testing results. Field diagnosis combined with centralized molecular resistance testing could improve typhoid management and surveillance in low-resource settings.

Assuntos

Técnicas Bacteriológicas/métodos; Testes Diagnósticos de Rotina/métodos; Farmacorresistência Bacteriana; Salmonella typhi/efeitos dos fármacos; Salmonella typhi/isolamento & purificação; Febre Tifoide/diagnóstico; Febre Tifoide/microbiologia; Adolescente; Adulto; Idoso; Idoso de 80 Anos ou mais; Antibacterianos/farmacologia; Criança; Pré-Escolar; DNA Girase/genética; DNA Bacteriano/genética; Feminino; Fluoroquinolonas/farmacologia; Humanos; Lactente; Recém-Nascido; Masculino; Pessoa de Meia-Idade; Técnicas de Diagnóstico Molecular/métodos; Projetos Piloto; Reação em Cadeia da Polimerase; Estudos Prospectivos; Fatores de Tempo; Adulto Jovem

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Salmonella typhi / Febre Tifoide / Técnicas Bacteriológicas / Farmacorresistência Bacteriana / Testes Diagnósticos de Rotina Tipo de estudo: Clinical_trials / Diagnostic_studies / Evaluation_studies / Observational_studies / Prognostic_studies Limite: Adolescent / Adult / Aged / Aged80 / Child / Child, preschool / Female / Humans / Infant / Male Idioma: En Ano de publicação: 2015 Tipo de documento: Article

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