[Carbohydrate metabolism and lactic acid biosynthesis of Lactococcus lactis subsp. lactis KLDS4.0325].

ABSTRACT

OBJECTIVE: We aimed to study the carbohydrate metabolism and lactic acid biosynthesis of Lactococcus lactis KLDS4. 0325. METHODS: Whole genome shot gun strategy was used for genome sequencing of strain L. lactis KLDS 4. 0325. Then, using bioinformatics method, we compared a series of protein-coding genes involved in transporting extracellular carbohydrate, sugar metabolism and lactic acid biosynthesis of strain L. lactis KLDS4. 0325 with other 9 reference strains. RESULTS: In L. lactis KLDS4. 0325 genome, where possesses more key enzyme coding genes related to the whole pathway of sugar metabolism than reference strains. CONCLUSION: In gene level, therefore, strain L. lactis KLDS4. 0325 shows a remarkable characteristic by utilizing various sugar to produce lactic acid, is a lactic acid bacteria with industrial potential of high yield L-lactic acid.