Characterisation of subpopulations of myeloid cells in infantile haemangioma.
J Clin Pathol
; 68(7): 571-4, 2015 Jul.
Article
em En
| MEDLINE
| ID: mdl-25834091
ABSTRACT
AIMS:
Cells expressing markers of mast cells, macrophages and dendritic cells have previously been demonstrated within the interstitium of infantile haemangioma (IH). This study characterised these myeloid cellular subpopulations within IH.METHODS:
Immunohistochemical staining was performed on proliferating and involuted IHs for the expression of Nanog, tryptase, CD163, DC-SIGN and CD45. The presence of mRNA corresponding to Nanog, tryptase α/ß-1, tryptase ß-2, CD163 and DC-SIGN was confirmed by NanoString and RT-PCR in snap-frozen IH tissues.RESULTS:
Immunohistochemical staining showed expression of Nanog by interstitial phenotypical mast cells within proliferating IH, which were separate from the interstitial M2-polarised macrophages that also expressed DC-SIGN, a dendritic cell marker. These two myeloid cellular subpopulations in IH did not express the pan-haematopoietic marker, CD45.CONCLUSIONS:
There are two interstitial subpopulations of myeloid cells within IH phenotypical mast cells which also express Nanog, indicating a primitive phenotype; and M2-polarised macrophages which also express DC-SIGN.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biomarcadores Tumorais
/
Hemangioma
/
Macrófagos
/
Mastócitos
Limite:
Child
/
Humans
/
Infant
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article